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banner Search results for Histone H3.1

Histone H3 Antibody (Pan) (clone RM188)

Catalog No : R20252
Applications : WB, ELISA, ICC
Reactivity : All Species
Format : Purified
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Western blot of recombinant Histone H3.3 and H3.1 proteins, A375, HEK293, HeLa and SK-MEL-2 whole cell lysates, using recombinant Histone H3 antibody at 0.025 ug/ml.
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Histone H3 Antibody (pS10) (clone PHH3/471R)

Catalog No : V8647
Applications : IHC-P
Reactivity : Human
Format : Purified
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IHC staining of FFPE human colon with recombinant PHH3 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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Histone H3 Antibody (pS10) (clone rPHH3/6824)

Catalog No : V9213
Applications : IHC-P, WB
Reactivity : Human
Format : Purified
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Western blot testing of human MCF7 cell lysate with phospho-Histone H3 antibody.
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Histone H3 Antibody (pS10) (clone rHH3/9924)

Catalog No : V5817
Applications : IHC-P, WB
Reactivity : Human
Format : Purified
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IHC staining of FFPE human tonsil tissue with Phosphorylated Histone H3 antibody (clone rHH3/9924). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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Histone H3 (acetyl K14) Antibody / HIST1H3A (clone BID-8)

Catalog No : FY12021
Applications : WB, IHC, ICC, IF, IP
Reactivity : Human, Mouse, Rat
Format : Liquid
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IHC analysis of Histone H3 (acetyl K14) using anti-Histone H3 (acetyl K14) antibody. Histone H3 (acetyl K14) was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with a dilution of 1:50 rabbit anti-Histone H3 (acetyl K14) antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.
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Histone H3 (mono methyl K14) Antibody / HIST1H3A (clone 31H98)

Catalog No : FY12032
Applications : WB, ICC, IF, FACS
Reactivity : Human, Mouse
Format : Liquid
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All lanes use the antibody at 1:1K dilution for 1 hour at room temperature.
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EMC4 Antibody / ER membrane protein complex subunit 4 (clone 30E32)

Catalog No : FY12033
Applications : WB, IHC, IP
Reactivity : Human, Mouse, Rat
Format : Liquid
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Immunoprecipitate (IP) analysis using the antibody at 1:50 dilution. (Western blot at 1:500 dilution). Predicted molecular weight: 15-20 kDa (three isoforms).
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Phospho-Histone H3 (Ser10) Antibody / HIST1H3A (clone 31H82)

Catalog No : FY12171
Applications : WB, IHC, ICC, IF
Reactivity : Human, Mouse, Rat
Format : Liquid
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Western blot analysis of Phospho-Histone H3 (S10) expression in HeLa treated Colcemid cell lysate, using Phospho-Histone H3 (Ser10) antibody.
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Phospho-Histone H3 (Ser28) Antibody / HIST1H3A (clone 32H09)

Catalog No : FY12176
Applications : WB, IHC, ICC, IF
Reactivity : Human, Mouse, Rat
Format : Liquid
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All lanes use the Phospho-Histone H3 (Ser28) antibody at 1:2K dilution for 1 hour at room temperature.
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Histone H3 (mono methyl K36) Antibody / HIST1H3A (clone DEG-8)

Catalog No : FY12310
Applications : WB, ICC, IF
Reactivity : Human, Mouse, Rat
Format : Liquid
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Western blot analysis of Histone H3 using Histone H3 (mono methyl K36) antibody. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Histone H3 antibody at 1:1000 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A specific band was detected for Histone H3 at approximately 15-17 kDa. The expected molecular weight of Histone H3 is at 15 kDa.
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