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Home >> Antibodies >> Zebrafish Srsf3 Antibody / Serine/arginine-rich splicing factor 3 / Isoforms a & b

Zebrafish Srsf3 Antibody / Serine/arginine-rich splicing factor 3 / Isoforms a & b (RZ1079)

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  Catalog No Formulation Size Price (USD)  
Image RZ1079 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 539
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IHC staining of FFPE zebrafish brain tissue with Zebrafish Srsf3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE zebrafish brain tissue with Zebrafish Srsf3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot analysis of Srsf3a/b protein using Zebrafish Srsf3 antibody and 1) zebrafish head, 2) whole female zebrafish and 3) whole male zebrafish tissue lysate. Predicted molecular weight: 19-20 kDa.
IHC staining of FFPE zebrafish colon tissue with Zebrafish Srsf3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE zebrafish skeletal muscle tissue with Zebrafish Srsf3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Availability 2-3 weeks
Species Reactivity Zebrafish
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity chromatography
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q801U3 , A5WWG4
Localization Nuclear, cytoplasmic
Applications Western Blot : 0.5-1 ug/ml
Immunohistochemistry (FFPE) : 2-5 ug/ml
Limitations This Zebrafish Srsf3 antibody is available for research use only.
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Description

Predicted to enable mRNA binding activity. Predicted to be involved in mRNA cis splicing, via spliceosome. Predicted to act upstream of or within RNA splicing and mRNA processing. Predicted to be active in nuclear speck. Orthologous to human SRSF3 (serine and arginine rich splicing factor 3).

Application Notes

Optimal dilution of the Zebrafish Srsf3 antibody should be determined by the researcher.

Immunogen

An E.coli-derived zebrafish Srsf3a/b recombinant protein (amino acids P4-A33) was used as the immunogen for the Zebrafish Srsf3 antibody. This antibody will detect the a and b isoforms.

Storage

After reconstitution, the Zebrafish Srsf3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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