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Home >> Antibodies >> Zebrafish ATP binding cassette E1 Antibody / Abce1

Zebrafish ATP binding cassette E1 Antibody / Abce1 (RZ1144)

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  Catalog No Formulation Size Price (USD)  
Image RZ1144 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 539
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IHC staining of FFPE zebrafish brain tissue with Zebrafish ATP binding cassette E1 antibody, HRP-secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE zebrafish brain tissue with Zebrafish ATP binding cassette E1 antibody, HRP-secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot analysis of Zebrafish ATP binding cassette E1 protein using Zebrafish ATP binding cassette E1 antibody and zebrafish embryo tissue lysate. Predicted molecular weight ~67 kDa.
IHC staining of FFPE zebrafish eye tissue with Zebrafish ATP binding cassette E1 antibody, HRP-secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Availability 2-3 weeks
Species Reactivity Zebrafish
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity chromatography
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q6TNW3
Applications Western Blot : 0.5-1 ug/ml
Immunohistochemistry (FFPE) : 2-5 ug/ml
Limitations This Zebrafish ATP binding cassette E1 antibody is available for research use only.
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Description

ATP binding cassette E1 (ABCE1, also RNase L inhibitor) is an ATPase found in humans involved in viral assembly. It is a member of the ATP-binding cassette (ABC) transporters superfamily and OABP subfamily. ABCE1 inhibits the action of ribonuclease L. Ribonuclease L normally binds to 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) and inhibits the interferon-regulated 2-5A/RNase L pathway, which is used by viruses. ABCE1 heterodimerize with ribonuclease L and prevents its interaction with 2-5A, antagonizing the anti-viral properties of ribonuclease L, and allow the virus to synthesize viral proteins. It has also been implicated to have an effect in tumorcell proliferation and antiapoptosis.

Application Notes

Optimal dilution of the Zebrafish ATP binding cassette E1 antibody should be determined by the researcher.

Immunogen

An E.coli-derived zebrafish ABCE1 recombinant protein (amino acids Q141-D599) was used as the immunogen for the Zebrafish ATP binding cassette E1 antibody.

Storage

After reconstitution, the Zebrafish ATP binding cassette E1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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