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Home >> Antibodies >> YWHAQ Antibody / 14-3-3 theta

YWHAQ Antibody / 14-3-3 theta [clone HHF-25] (RQ8915)

  Catalog No Formulation Size Price (USD)  
Image RQ8915 Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg/ml BSA 100 ul 439
Microvalidated Recrabbitmono
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IHC staining of FFPE human pancreatic cancer tissue with YWHAQ antibody, HRP-secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human HeLa, 2) human A549, 3) human SH-SY5Y, 4) human U-251, 5) rat brain, 6) rat C6 and 7) mouse brain tissue lysate with YWHAZ antibody. Predicted molecular weight ~28 kDa.
Availability 1-3 days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name HHF-25
Purity Affinity chromatography
UniProt P27348
Localization Cytoplasm
Applications Western Blot : 1:500
Immunohistochemistry (FFPE) : 1:50
Limitations This YWHAQ antibody is available for research use only.
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  • Applications : WB, ELISA (peptide)
    Reactivity : Human
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Description

YWHAQ, also known as 14-3-3 theta, is a member of the 14-3-3 protein family that regulates diverse cellular processes including signal transduction, apoptosis, and cell cycle control. It functions as an adaptor protein, binding to phosphorylated partners to influence their localization, stability, and activity. Researchers commonly use a YWHAQ antibody to study these signaling networks.

YWHAQ is widely expressed in human tissues and has been linked to neuronal function, stress responses, and cancer progression. Its interaction with kinases, transcription factors, and cytoskeletal proteins makes it an important regulator of cellular homeostasis. Employing a YWHAQ antibody enables detailed investigation of its role in pathways controlling growth, survival, and differentiation.

NSJ Bioreagents offers a high-quality YWHAQ antibody validated for western blot, immunohistochemistry, and immunoprecipitation. Selecting the right YWHAQ antibody ensures sensitive and reproducible detection in studies of cell signaling, neurobiology, and disease research.

Application Notes

Optimal dilution of the YWHAQ antibody should be determined by the researcher.

Immunogen

A peptide sequence specific to 14-3-3 theta protein was used as the immunogen for the YWHAQ antibody.

Storage

After reconstitution, the YWHAQ antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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