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Home >> Antibodies >> Recombinant p27 Antibody / p27Kip1

Recombinant p27 Antibody / p27Kip1 [clone KIP1/9168R] (V5554)

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  Catalog No Formulation Size Price (USD)  
Image V5554-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 539
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V5554-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 249
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V5554SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
Microvalidated Recrabbitmono
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IHC staining of FFPE human prostate tissue. Strong nuclear staining observed using recombinant p27 antibody at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human prostate tissue. Strong nuclear staining observed using recombinant p27 antibody at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant p27 antibody (clone KIP1/9168R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name KIP1/9168R
Purity Protein A/G affinity
UniProt P46527
Localization Nucleus
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant p27 antibody is available for research use only.
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Description

p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF-b induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix.

Application Notes

Optimal dilution of the recombinant p27 antibody should be determined by the researcher.

Immunogen

Recombinant full-length human CDKN1B/p27Kip1 protein was used as the immunogen for the recombinant p27 antibody.

Storage

Aliquot the recombinant p27 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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