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Home >> Antibodies >> Recombinant CR2 Antibody / Complement receptor 2 / CD21

Recombinant CR2 Antibody / Complement receptor 2 / CD21 [clone rCR2/9537] (V5514)

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  Catalog No Formulation Size Price (USD)  
Image V5514-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 539
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V5514-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 249
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V5514SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
Microvalidated Recmousemono
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IHC staining of FFPE human tonsil tissue with recombinant CR2 antibody (clone rCR2/9537). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human tonsil tissue with recombinant CR2 antibody (clone rCR2/9537). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant CR2 antibody (clone rCR2/9537) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG2b, kappa
Clone Name rCR2/9537
Purity Protein A/G affinity
UniProt P20023
Localization Cell membrane
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant CR2 antibody is available for research use only.
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Description

Recognizes a protein of 140kDa, which is identified as the complement receptor 2(CR2)/CD21. This protein is expressed strongly on mature B cells, follicular dendritic cells and weakly on immature thymocytes and T lymphocytes. In B-cell ontogeny, CD21 appears after the pre-B-stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells. CD21 expression is also gradually lost after stimulation of B cells in vitro.CD21 functions as receptor for C3d, C3dg and iC3b Complement components, for EBV and for IFNalpha.CD21 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B cell activation.

Application Notes

Optimal dilution of the recombinant CR2 antibody should be determined by the researcher.

Immunogen

A recombinant fragment (within amino acids 100-300) of human Complement receptor 2/CD21 protein was used as the immunogen for the recombinant CR2 antibody.

Storage

Aliquot the recombinant CR2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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