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Home >> Antibodies >> Recombinant CDKN1B Antibody / p27Kip1

Recombinant CDKN1B Antibody / p27Kip1 [clone rCDKN1B/13245] (V5645)

  Catalog No Formulation Size Price (USD)  
Image V5645-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 539
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V5645-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 249
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V5645SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
Microvalidated Recmousemono
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Western blot testing of human SK-BR-3 cell lysate with recombinant CDKN1B antibody (clone rCDKN1B/13245). Commonly observed at ~27 kDa.
IHC staining of FFPE human placental tissue with recombinant CDKN1B antibody (clone PASE/4LJ). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant CDKN1B antibody (clone rCDKN1B/13245) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG2a, kappa
Clone Name rCDKN1B/13245
Purity Protein G affinity
UniProt P46527
Localization Cytoplasm, Endosome, Nucleus
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Western Blot : 2-4ug/ml
Limitations This recombinant CDKN1B antibody is available for research use only.
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Description

This MAb recognizes a 27kDa protein called p27Kip1/CDKN1B/Cyclin-dependent kinase inhibitor 1B, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This MAb is excellent for staining of formalin-fixed tissues.

Application Notes

Optimal dilution of the recombinant CDKN1B antibody should be determined by the researcher.

Immunogen

A recombinant human CDKN1B protein was used as the immunogen for the recombinant CDKN1B antibody.

Storage

Aliquot the recombinant CDKN1B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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