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Home >> Antibodies >> PRRC1 Antibody / Proline-rich and coiled-coil-containing protein 1

PRRC1 Antibody / Proline-rich and coiled-coil-containing protein 1 (RQ8248)

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  Catalog No Formulation Size Price (USD)  
Image RQ8248 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 439
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Immunofluorescent staining of FFPE human HeLa cells with PRRC1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human HeLa cells with PRRC1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HeLa, 2) MCF7, 3) HepG2 and 4) Caco-2 cell lysate with PRRC1 antibody. Predicted molecular weight ~47 kDa.
Flow cytometry testing of fixed and permeabilized human U-251 cells with PRRC1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PRRC1 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q96M27
Localization Cytoplasm
Applications Western Blot : 0.5-1ug/ml
Immunofluorescence : 5ug/ml
Flow Cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This PRRC1 antibody is available for research use only.
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Description

PRRC1 (Proline-Rich Coiled-Coil 1) is a Protein Coding gene. The PRRC1 gene, located on 5q23.2, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, and frog. The PRCC1 gene has two distinct regions: a proline-rich region on the N-terminus, and the DUF84 region on the C-terminus. PRCC1 is the commonly identified protein name of CAD38605. It encodes for 445 amino acids for a predicted total of 6 exons. The predicted molecular weight is 46.7 kDa, and the isoelectric point is 5.46. Orthologs have been determined in most eukaryotes, the most highly conserved being found in most mammalian species. PRRC1 is widely expressed in the thyroid, endometrium, and other tissues. Diseases associated with PRRC1 include Dystonia 23.

Application Notes

Optimal dilution of the PRRC1 antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids Y139-D411) was used as the immunogen for the PRRC1 antibody.

Storage

After reconstitution, the PRRC1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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