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Home >> Antibodies >> INTS12 Antibody / Integrator complex subunit 12

INTS12 Antibody / Integrator complex subunit 12 (RQ8807)

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  Catalog No Formulation Size Price (USD)  
Image RQ8807 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 439
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Flow cytometry testing of fixed and permeabilized human MCF7 cells with INTS12 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= INTS12 antibody.
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Flow cytometry testing of fixed and permeabilized human MCF7 cells with INTS12 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= INTS12 antibody.
Western blot testing of 1) human HepG2, 2) human K562, 3) human Jurkat, 4) human HEL, 5) rat PC-12, 6) rat NRK, 7) mouse RAW264.7 and 8) mouse SP2/0 cell lysate with INTS12 antibody. Predicted molecular weight ~49 kDa but may be observed at higher molecular weights due to sumoylation and phosphorylation.
Availability 1-3 days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity chromatography
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q96CB8
Applications Western Blot : 1-2ug/ml
Flow Cytometry : 1-3ug/million cells
ELISA : 0.1-0.5ug/ml
Limitations This INTS12 antibody is available for research use only.
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Description

Component of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. [UniProt]

Application Notes

Optimal dilution of the INTS12 antibody should be determined by the researcher.

Immunogen

An E.coli-derived human recombinant protein (amino acids M1-K384) was used as the immunogen for the INTS12 antibody.

Storage

After reconstitution, the INTS12 Antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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