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Home >> Antibodies >> Gp36 Antibody / Glycoprotein 36 / Podoplanin

Gp36 Antibody / Glycoprotein 36 / Podoplanin [clone PDPN/7808R] (V4700)

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  Catalog No Formulation Size Price (USD)  
Image V4700-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 539
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V4700-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 249
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V4700SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
Microvalidated Recrabbitmono
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Western blot testing of human lung tissue lysate with Gp36 antibody. Predicted molecular weight ~25 kDa but may be observed at higher molecular weights due to glycosylation.
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Western blot testing of human lung tissue lysate with Gp36 antibody. Predicted molecular weight ~25 kDa but may be observed at higher molecular weights due to glycosylation.
IHC staining of FFPE human tonsil tissue with Gp36 antibody (clone PDPN/7808R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name PDPN/7808R
Purity Protein A/G affinity
UniProt Q86YL7
Localization Cell surface
Applications ELISA (Order BSA-free Format For Coating) :
Western Blot : 2-4ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This Gp36 antibody is available for research use only.
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Description

It recognizes a muco-protein of 38-43kDa, which is identified as Podoplanin (PDPN), also called Glycoprotein 36 (Gp36). It localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. It is selectively expressed in lymphatic endotheliumas well as lymphoangiomas, Kaposi sarcomas, and in a subset of angiosarcomas with probable lymphatic differentiation. Recent studies have also shown podoplanin to be a highly sensitive and relatively specific marker for epithelioid mesothelioma. Therefore, it can be used in a panel to distinguish mesotheliomas or mesothelial cells from pulmonary carcinomas.

Application Notes

Optimal dilution of the Gp36 antibody should be determined by the researcher.

Immunogen

A recombinant partial protein sequence (within amino acids 1-100) from the human protein was used as the immunogen for the Gp36 antibody.

Storage

Aliquot the Gp36 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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