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Home >> Antibodies >> Elastase 3A Antibody / CELA3A

Elastase 3A Antibody / CELA3A [clone CELA3A/12552] (V5637)

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  Catalog No Formulation Size Price (USD)  
Image V5637-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 539
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V5637-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 249
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V5637SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
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Western blot testing of human pancreas tissue lysate with Elastase 3A antibody (clone CELA3A/12552). Predicted molecular weight ~29 kDa.
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Western blot testing of human pancreas tissue lysate with Elastase 3A antibody (clone CELA3A/12552). Predicted molecular weight ~29 kDa.
IHC staining of FFPE human pancreas tissue with Elastase 3A antibody (clone CELA3A/12552). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Elastase 3A antibody (clone CELA3A/12552) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name CELA3A/12552
Purity Protein G affinity
UniProt P09093
Localization Cytoplasm, Extracellular space
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Western Blot : 1-2ug/ml
Limitations This Elastase 3A antibody is available for research use only.
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Description

Elastases belong to a subfamily of serine proteases that function to catalytically hydrolyze a wide variety of proteins. Elastase-3A, also known as ELA3A or ELA3, is a 270 amino acid protein that belongs to the elastase family and acts as an alanine-specific protease. Secreted as a zymogen from the pancreas, Elastase-3A functions as a digestive enzyme in the intestine that participates in metabolic degradation pathways by preferentially cleaving proteins that have alanine residues. In addition, Elastase-3A is thought to play a role in the metabolism and transport of cholesterol molecules in the intestine. Altered expression of Elastase-3A may be associated with pancreatic ductal carcinoma, a highly fatal cancer of the pancreatic ducts

Application Notes

Optimal dilution of the Elastase 3A antibody should be determined by the researcher.

Immunogen

A portion of amino acids 50-250 from human CELA3A protein was used as the immunogen for the Elastase 3A antibody.

Storage

Aliquot the Elastase 3A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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