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Home >> Antibodies >> Cytokeratin CAM5.2 Antibody / Cytokeratin 7/8

Cytokeratin CAM5.2 Antibody / Cytokeratin 7/8 [clone CAM5.2] (V5837)

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  Catalog No Formulation Size Price (USD)  
Image V5837-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 539
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V5837-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 249
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V5837SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
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IHC staining of FFPE human breast carcinoma tissue with Cytokeratin CAM5.2 antibody (clone CAM5.2). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast carcinoma tissue with Cytokeratin CAM5.2 antibody (clone CAM5.2). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a, kappa
Clone Name CAM5.2
Purity Protein G affinity
Localization Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This Cytokeratin CAM5.2 antibody is available for research use only.
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Description

Cytokeratin CAM5.2 antibody has a primary reactivity with human keratin proteins that correspond to Moll’s peptides #7 and #8, MW 48 and 52 kDa, respectively. Cytokeratin 7 and 8 are present in secretory epithelia of normal human tissue but not on stratified squamous epithelium.

Cytokeratin LMW CAM5.2 antibody stains most epithelial-derived tissue, including liver, renal tubular epithelium, and hepatocellular and renal cell carcinomas. Anti-Cytokeratin (CAM 5.2) may not react with some squamous cell carcinomas.

Application Notes

Optimal dilution of the Cytokeratin CAM5.2 antibody should be determined by the researcher.

Immunogen

The human colorectal carcinoma cell line HT29 was used as the immunogen for the was used as the immunogen for the Cytokeratin CAM5.2 antibody.

Storage

Aliquot the Cytokeratin CAM5.2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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