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Home >> Antibodies >> b-Actin Antibody

b-Actin Antibody [clone 137CT26.1.1] (F40216)

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  Catalog No Formulation Size Price (USD)  
Image F40216-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 429
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F40216-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 199
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Western blot analysis of b-Actin antibody in 1) human K562, 2) human HL-60, 3) human HeLa cell line, and mouse tissues 4) spleen, 5) liver, 6) mouse NIH3T3 cell lysate, 7) mouse cerebellum and 8) mouse brain tissue lysate. Predicted molecular weight ~42 kDa.
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Western blot analysis of b-Actin antibody in 1) human K562, 2) human HL-60, 3) human HeLa cell line, and mouse tissues 4) spleen, 5) liver, 6) mouse NIH3T3 cell lysate, 7) mouse cerebellum and 8) mouse brain tissue lysate. Predicted molecular weight ~42 kDa.
Immunohistochemical analysis of paraffin-embedded human spleen using b-Actin antibody at 1:25 dilution.
Confocal immunofluorescent analysis of b-Actin antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used as a nuclear counterstain (blue).
Flow cytometry testing of human HeLa cells with b-Actin antibody; Blue=isotype control, Green= b-Actin antibody.
Western blot testing of 1) human A431, 2) rat C2C12, 3) rat C6, 4) human HeLa and 5) human MCF7 cell lysate with b-Actin antibody. Predicted molecular weight ~42 kDa.
Western blot testing of 1) human HeLa, 2) human HepG2 and 3) mouse NIH 3T3 cell lysate with b-Actin antibody. Predicted molecular weight ~42 kDa.
IHC staining of FFPE human skeletal muscle tissue with b-Actin antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC staining of FFPE human heart tissue with b-Actin antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Predicted Reactivity Bovine, Chicken, Horse, Pig, Primate, Sheep, Xenopus
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, k
Clone Name 137CT26.1.1
Purity Purified
UniProt P60709
Applications Immunohistochemistry (Paraffin) : 1:10-1:50
Immunofluorescence : 1:10-1:50
Western Blot : 1:1000
Flow Cytometry : 1:25
Limitations This b-Actin antibody is available for research use only.
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Description

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus. It is classically referred to as being a 'nonmuscle' actin, however expression is ubiquitous, strongly in lymph tissue and weakly in heart and muscle.

Application Notes

Titration of the b-Actin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

This b-Actin antibody was produced from a mouse immunized with ACTB recombinant protein.

Storage

Aliquot the b-Actin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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