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banner Search results for Cathepsin F

CTSF Antibody / Cathepsin F

Catalog No : FY13174
Applications : WB, IHC, FACS
Reactivity : Human
Format : Lyophilized
Immunohistochemical staining of Cathepsin F/CTSF using anti-CTSF antibody. Cathepsin F/CTSF was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-CTSF antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.
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CTSF Antibody / Cathepsin F

Catalog No : R33242
Applications : WB, ELISA (peptide)
Reactivity : Human
Pred. Reactivity :Mouse, Rat, Dog
Format : Antigen affinity purified
Western blot testing of human ovay lysate with Cathepsin F antibody at 0.3ug/ml. CTSF is synthesized as a ~53 kDa precursor; processing is described (Jerič et al., 2013) and smaller fragments are reported, though published WB images with ~30-35 kDa bands are limited. The ~30-35 kDa species observed here is therefore consistent with a mature or trimmed CTSF form, but has less direct precedent in the literature than the non-processed band.
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