TTF-1 Antibody / NKX2.1 [clone 8G7G3/1] (V2274)

Description

TTF-1 antibody clone 8G7G3/1 is a monoclonal antibody that recognizes thyroid transcription factor 1, also called NKX2.1, a nuclear transcription factor critical for development and function of the thyroid, lung, and brain. TTF-1 regulates the expression of thyroid-specific proteins such as thyroglobulin and thyroperoxidase, as well as surfactant proteins in lung epithelial cells. Because of its restricted expression pattern, TTF-1 is a valuable marker in both developmental biology and tumor pathology. NSJ Bioreagents supplies TTF-1 antibody clone 8G7G3/1 for researchers studying tissue-specific gene regulation, organogenesis, and cancer.

In pathology, TTF-1 antibody clone 8G7G3/1 is widely used to determine tumor origin. Its nuclear staining in lung adenocarcinomas and thyroid carcinomas makes it an essential diagnostic marker. Pathologists apply this antibody to distinguish primary lung tumors from metastatic carcinomas, as well as to identify thyroid origin in metastatic disease. The clear nuclear signal produced by clone 8G7G3/1 provides high specificity for these tissue types.

In developmental biology, TTF-1 antibody clone 8G7G3/1 has been used to investigate the role of NKX2.1 in organogenesis. TTF-1 is essential for thyroid follicular cell differentiation, lung branching morphogenesis, and certain aspects of forebrain development. This antibody allows researchers to track TTF-1 expression during embryogenesis, clarifying how transcription factors orchestrate organ development.

In cancer research, TTF-1 antibody clone 8G7G3/1 has been employed in molecular studies of tumor progression and lineage determination. Expression of TTF-1 in lung adenocarcinoma is associated with particular genetic subtypes, making it useful for refining classification and predicting therapeutic responses. Its absence in squamous cell carcinoma of the lung further enhances its diagnostic specificity.

TTF-1 antibody clone 8G7G3/1 has been validated in tissue and cell-based studies, providing consistent nuclear staining with minimal background. It has an extensive publication record spanning pathology, developmental biology, and oncology. Alternate names include thyroid transcription factor 1 antibody, NKX2.1 antibody, and thyroid-lung lineage transcription factor antibody.

Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TTF-1 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Rat full length recombinant protein was used as the immunogen for this TTF-1 antibody.

Storage

Store the TTF-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).