Zebrafish Tgfb1 Antibody / Tgfb1a / Tgf beta (RZ1180)

Description

Transforming Growth Factor-beta (TGF-beta) is a multifunctional cytokine that regulates a wide array of biological processes, including cell proliferation, differentiation, apoptosis, and extracellular matrix production. In zebrafish (Danio rerio), multiple tgfb genes have been identified, encoding proteins that are structurally and functionally conserved with their mammalian counterparts.

Zebrafish TGF-beta proteins belong to the TGF-beta superfamily, which also includes activins, nodal, and bone morphogenetic proteins (BMPs). These ligands signal through serine/threonine kinase receptors, leading to the phosphorylation of receptor-regulated SMADs (R-Smads), which then form complexes with co-Smad (Smad4) and translocate to the nucleus to regulate gene expression.

TGF-beta signaling in zebrafish is critical during embryogenesis and tissue morphogenesis. It plays essential roles in mesoderm formation, left-right axis specification, cardiovascular development, and immune system modulation. Specific zebrafish isoforms, such as tgfb1a, tgfb1b, and tgfb3, exhibit distinct expression patterns and developmental functions. For example, tgfb1b is involved in hematopoiesis and immune regulation, while tgfb3 contributes to craniofacial development and epithelial integrity.

Due to the genetic tractability and optical transparency of zebrafish embryos, this model system provides unique advantages for in vivo analysis of TGF-β pathway dynamics. Zebrafish TGF-beta proteins serve as valuable tools for studying developmental biology, regeneration, fibrosis, and disease mechanisms, including cancer and immune dysfunction.

Application Notes

Optimal dilution of the Zebrafish Tgfb1 antibody should be determined by the researcher.

Immunogen

E. coli-derived zebrafish Tgfb1 recombinant protein (amino acids A266-S377) was used as the immunogen for the Zebrafish Tgfb1 antibody.

Storage

After reconstitution, the Zebrafish Tgfb1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.