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Home >> Antibodies >> Zebrafish Srsf1 Antibody / Serine/arginine-rich splicing factor 1 / Isoforms a & b

Zebrafish Srsf1 Antibody / Serine/arginine-rich splicing factor 1 / Isoforms a & b (RZ1023)

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Image RZ1023 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 539
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Immunohistochemical analysis of Srsf1a/b protein using Zebrafish Srsf1 antibody and paraffin-embedded zebrafish brain tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Immunohistochemical analysis of Srsf1a/b protein using Zebrafish Srsf1 antibody and paraffin-embedded zebrafish brain tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot analysis of Srsf1a/b protein using Zebrafish Srsf1 antibody and zebrafish embryo tissue lysate. The predicted molecular weight of Srsf1a/b is 28 kDa.
Immunohistochemical analysis of Srsf1a/b protein using Zebrafish Srsf1 antibody and paraffin-embedded zebrafish colon tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunohistochemical analysis of Srsf1a/b protein using Zebrafish Srsf1 antibody and paraffin-embedded zebrafish kidney tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunohistochemical analysis of Srsf1a/b protein using Zebrafish Srsf1 antibody and paraffin-embedded zebrafish spinal cord tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunohistochemical analysis of Srsf1a/b protein using Zebrafish Srsf1 antibody and paraffin-embedded zebrafish eye tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunohistochemical analysis of Srsf1a/b protein using Zebrafish Srsf1 antibody and paraffin-embedded zebrafish skeletal muscle tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Availability 2-3 weeks
Species Reactivity Zebrafish
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity chromatography
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q7SXP4 , Q6NYA0
Localization Nuclear, cytoplasmic
Applications Western Blot : 0.5-1 ug/ml
Immunohistochemistry (FFPE) : 2-5 ug/ml
Limitations This Zebrafish Srsf1 antibody is available for research use only.
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Description

Predicted to enable mRNA binding activity. Predicted to be involved in alternative mRNA splicing, via spliceosome. Predicted to act upstream of or within RNA splicing and mRNA processing. Predicted to be located in nucleus. Predicted to be active in nuclear speck. Human ortholog(s) of this gene implicated in neurodevelopmental disorder with dysmorphic facies and behavioral abnormalities. Orthologous to human SRSF1 (serine and arginine rich splicing factor 1).

Application Notes

Optimal dilution of the Zebrafish Srsf1 antibody should be determined by the researcher.

Immunogen

An E.coli-derived zebrafish recombinant protein (amino acids S2-E32) was used as the immunogen for the Zebrafish Srsf1 antibody. This antibody will detect the a and b isoforms.

Storage

After reconstitution, the Zebrafish Srsf1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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