Zebrafish Shha Antibody / Shh / Sonic Hedgehog (RZ1309)

Description

Zebrafish Shha, also known as sonic hedgehog a, is a signaling molecule that plays a critical role in embryonic development, pattern formation, and cell differentiation. Shha belongs to the hedgehog family of secreted proteins that regulate key developmental pathways, including neural tube patterning, somite organization, and craniofacial morphogenesis. It is particularly important for the proper formation of the central nervous system, muscle segmentation, and the shaping of the midline structures during early development.

Zebrafish Shha is an ortholog of the human sonic hedgehog (SHH) protein and shares a high level of sequence and functional conservation. Like human SHH, zebrafish Shha undergoes proteolytic processing and lipid modification to produce an active signaling molecule that interacts with the Patched receptor and initiates downstream signaling through the Smoothened and Gli transcription factors. This pathway regulates the expression of numerous target genes that influence cell fate and tissue organization.

There are no known isoforms of zebrafish Shha, but zebrafish possess another related hedgehog gene called Shhb, which shares overlapping but distinct roles during development. The combination of Shha and Shhb activity helps orchestrate the complex patterning events required for normal embryogenesis.

Antibodies against zebrafish Shha protein are valuable tools for studying hedgehog signaling pathways, developmental biology, and tissue patterning. These antibodies can be applied in techniques such as western blot, immunohistochemistry, immunofluorescence, and ELISA to monitor Shha expression and localization in embryonic tissues.

Application Notes

Optimal dilution of the Zebrafish Shha antibody should be determined by the researcher.

Immunogen

E. coli-derived zebrafish Shha recombinant protein (amino acids Q208-H409) was used as the immunogen for the Zebrafish Shha antibody.

Storage

After reconstitution, the Zebrafish Shha antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.