Zebrafish Psmc5 Antibody / 26S proteasome regulatory subunit 8 (RZ1290)

Description

Psmc5 (Proteasome 26S subunit ATPase 5) is a crucial component of the 26S proteasome, which is involved in the ATP-dependent degradation of ubiquitinated proteins. As a member of the AAA ATPase family, Psmc5 plays a significant role in recognizing and unfolding substrates before they are translocated into the 20S catalytic core of the proteasome for proteolysis. This function is essential for maintaining cellular homeostasis by regulating protein turnover, cell cycle progression, and response to stress.

In zebrafish, Psmc5 is the ortholog of the human PSMC5 gene, and they share high sequence identity as well as functional conservation. The zebrafish and human proteins possess conserved domains critical for ATP hydrolysis and substrate processing, making zebrafish an excellent model for studying proteasome function.

Like its human counterpart, Psmc5 in zebrafish may have isoforms resulting from alternative splicing. These isoforms could exhibit differences in regulatory subunit composition or functional roles within the proteasomal complex, especially in specialized tissues or during developmental stages.

Expression of Psmc5 in zebrafish is widespread, with notable presence in actively dividing cells, such as those in the brain, heart, and digestive tissues. This distribution underscores its essential role in development and cellular maintenance.

In humans, mutations or dysregulation of PSMC5 have been associated with various diseases, including neurodegenerative disorders and cancers, often due to impaired proteasomal degradation. Zebrafish Psmc5 serves as an ideal model for studying proteasome-related diseases and for screening potential therapeutic agents that target the proteasome machinery.

Application Notes

Optimal dilution of the Zebrafish Psmc5 antibody should be determined by the researcher.

Immunogen

E. coli-derived zebrafish Psmc5 recombinant protein (amino acids N41-K393) was used as the immunogen for the Zebrafish Psmc5 antibody.

Storage

After reconstitution, the Zebrafish Psmc5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.