Zebrafish p53 Antibody / Tp53 / Tumor protein 53 (RZ1259)

Description

p53, also known as Tumor Protein 53, is a crucial tumor suppressor protein that plays an essential role in maintaining cellular integrity by regulating the cell cycle, promoting DNA repair, and initiating apoptosis in response to stress, including DNA damage, hypoxia, and oxidative stress. It acts as a transcription factor and regulates the expression of a variety of target genes involved in these processes, thus preventing the accumulation of genetic mutations that could lead to cancer.

In zebrafish, the p53 protein is an ortholog of the human TP53 gene, and both share highly conserved structural domains, including the DNA binding domain and the oligomerization domain, which are crucial for its function as a transcription factor. The zebrafish p53 protein shares many functional similarities with its human counterpart, including its ability to activate target genes like p21 and Bax in response to cellular stress and its involvement in apoptosis regulation. This makes zebrafish a valuable model for studying the role of p53 in cancer biology, DNA damage responses, and developmental processes.

During zebrafish development, p53 is involved in embryogenesis, cell differentiation, and tissue remodeling. p53’s function is particularly important in the early stages of zebrafish development, where it helps maintain genomic stability by responding to various stress signals. In cases of severe DNA damage or other cellular insults, p53 activation can lead to cell cycle arrest or apoptosis, which ensures that damaged cells do not proliferate, thereby preventing potential tumor formation.

The zebrafish model has been widely used in cancer research due to the ease of genetic manipulation and the similarity of key signaling pathways like the p53 pathway. p53 mutations in zebrafish, like in humans, can lead to abnormal cellular proliferation, tissue development defects, and tumor formation. Understanding the molecular pathways controlled by p53 in zebrafish is crucial for modeling human diseases such as cancer, neurodegeneration, and aging.

Given its evolutionary conservation and critical role in cell cycle regulation, apoptosis, and tumor suppression, zebrafish p53 is a powerful tool for studying oncogenesis, genomic stability, drug screening, and genetic disorders in vertebrate models.

Application Notes

Optimal dilution of the Zebrafish p53 antibody should be determined by the researcher.

Immunogen

E. coli-derived zebrafish Tumor protein 53 (amino acids D29-Q358) was used as the immunogen for the Zebrafish p53 antibody.

Storage

After reconstitution, the Zebrafish p53 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.