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Home >> Antibodies >> Zebrafish Mylz2 Antibody / Fast skeletal myosin light chain 2 / Mylpfa/b

Zebrafish Mylz2 Antibody / Fast skeletal myosin light chain 2 / Mylpfa/b (RZ1326)

  Catalog No Formulation Size Price (USD)  
Image RZ1326 0.5mg/ml in PBS with 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3. 200 ul 539
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IHC staining of FFPE zebrafish skeletal muscle tissue with Mylz2 antibody, HRP-labeled secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE zebrafish skeletal muscle tissue with Mylz2 antibody, HRP-labeled secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot analysis of Mylz2/Mylpfa protein using Zebrafish Mylz2 antibody and 1) zebrafish head, 2) whole female zebrafish, 3) whole male zebrafish and 4) zebrafish embryo tissue lysate. Predicted molecular weight ~19 kDa.
Availability 2-3 weeks
Species Reactivity Zebrafish
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity chromatography
UniProt O93409 , Q66I73
Localization Cytoplasm
Applications Western Blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Limitations This Zebrafish Mylz2 antibody is available for research use only.
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Description

Zebrafish Mylz2 (Fast skeletal myosin light chain 2), also called Mylpfa (Myosin regulatory light chain 2, skeletal muscle isoform A), is a regulatory component of the myosin complex that modulates actin-myosin interactions in fast-twitch skeletal muscle fibers. It is essential for muscle contraction, fiber stability, and efficient locomotor activity during zebrafish development and adulthood.

Mylz2 expression is restricted to fast skeletal muscle, making it a valuable marker for studying muscle specialization and performance. The human ortholog of Mylz2 is MYL1, which encodes myosin light chain 1/3, a protein involved in contractile regulation of human skeletal muscle. This conservation underscores its role in fundamental muscle biology across vertebrates.

Using a high-quality Zebrafish Mylz2 antibody enables reliable detection in assays such as western blot, immunohistochemistry, and immunofluorescence. A Zebrafish Mylz2 antibody from NSJ Bioreagents ensures sensitivity and reproducibility for studies in skeletal muscle development, contractile regulation, and comparative physiology. Selecting the right Zebrafish Mylz2 antibody is crucial for generating accurate and consistent results.

Application Notes

Optimal dilution of the Zebrafish Mylz2 antibody should be determined by the researcher.

Immunogen

The immunogen used for this Mylz2 antibody (Mylpfa) is 100% homologous with Mylpfb.

Storage

Store the Zebrafish Mylz2 antibody at -20oC.

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