Zebrafish Luteinizing hormone beta Antibody / Lhb (RZ1179)

Description

Luteinizing hormone (LH) is a glycoprotein hormone critical for the regulation of reproductive function in vertebrates. It consists of a common alpha subunit and a hormone-specific beta subunit, the latter conferring biological specificity. In zebrafish (Danio rerio), the Lhb gene encodes the beta subunit of LH and plays a key role in the hypothalamic-pituitary-gonadal (HPG) axis.

Zebrafish Lhb is produced by gonadotrope cells in the anterior pituitary and is essential for triggering final oocyte maturation and ovulation in females, as well as steroidogenesis and spermatogenesis in males. Its expression is tightly regulated by gonadotropin-releasing hormone (GnRH) and modulated by sex steroids through feedback mechanisms.

The zebrafish model has been instrumental in dissecting the molecular and developmental regulation of lhb expression. Notably, Lhb mRNA becomes detectable during larval stages and increases during sexual maturation. Functional studies using gene knockout and reporter lines have demonstrated that Lhb is indispensable for fertility, and its disruption results in reproductive failure.

Due to its conserved structure and function, zebrafish Lhb serves as a valuable model for studying reproductive endocrinology, pituitary cell differentiation, and hormone signaling pathways in vertebrates.

Application Notes

Optimal dilution of the Zebrafish Luteinizing hormone beta antibody should be determined by the researcher.

Immunogen

E. coli-derived zebrafish Luteinizing hormone beta recombinant protein (amino acids L24-Y140) was used as the immunogen for the Zebrafish Luteinizing hormone beta antibody.

Storage

After reconstitution, the Zebrafish Luteinizing hormone beta antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.