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Home >> Antibodies >> Zebrafish Klhl12 Antibody / Kelch-like protein 12

Zebrafish Klhl12 Antibody / Kelch-like protein 12 (RZ1009)

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Image RZ1009 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 539
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Western blot analysis of Klhl12 protein using Klhl12 antibody and zebrafish embryo tissue lysate. The predicted molecular weight of Klhl12 is ~63 kDa.
Western blot analysis of Klhl12 protein using Klhl12 antibody and zebrafish embryo tissue lysate. The predicted molecular weight of Klhl12 is ~63 kDa.
Availability 2-3 weeks
Species Reactivity Zebrafish
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity chromatography
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q5U374
Applications Western Blot : 0.5-1 ug/ml
Limitations This Zebrafish Klhl12 antibody is available for research use only.
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Description

Kelch-like protein 12 is a protein that in humans is encoded by the KLHL12 gene. This gene encodes a member of the KLHL (Kelch-like) family of proteins. This protein has been identified as an autoantigen in the autoimmune disease Sjogren's syndrome and as a potential biomarker in primary biliary cirrhosis. This protein may act as a substrate adaptor of the Cullin-3 ubiquitin ligase complex to promote substrate-specific ubiquitylation. Ubiquitylation by this complex has been shown to regulate the Wnt signaling pathway as well as COPII vesicle coat size. A pseudogene has been identified on chromosome 22. Alternative splicing results in multiple transcript variants.

Application Notes

Optimal dilution of the Zebrafish Klhl12 antibody should be determined by the researcher.

Immunogen

An E.coli-derived zebrafish Klhl12 recombinant protein (amino acids R23-V327) was used as the immunogen for the Zebrafish Klhl12 antibody.

Storage

After reconstitution, the Zebrafish Klhl12 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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