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Home >> Antibodies >> Zebrafish Idh3b Antibody

Zebrafish Idh3b Antibody (RZ1016)

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Image RZ1016 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 539
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Immunohistochemical analysis of Idh3b protein using Zebrafish Idh3b antibody and paraffin-embedded zebrafish pancreas tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Immunohistochemical analysis of Idh3b protein using Zebrafish Idh3b antibody and paraffin-embedded zebrafish pancreas tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunohistochemical analysis of Idh3b protein using Zebrafish Idh3b antibody and paraffin-embedded zebrafish liver tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunohistochemical analysis of Idh3b protein using Zebrafish Idh3b antibody and paraffin-embedded zebrafish heart tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunohistochemical analysis of Idh3b protein using Zebrafish Idh3b antibody and paraffin-embedded zebrafish muscle tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunohistochemical analysis of Idh3b protein using Zebrafish Idh3b antibody and paraffin-embedded zebrafish spinal cord tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunohistochemical analysis of Idh3b protein using Zebrafish Idh3b antibody and paraffin-embedded zebrafish cerebellum tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunohistochemical analysis of Idh3b protein using Zebrafish Idh3b antibody and paraffin-embedded zebrafish kidney tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Availability 2-3 weeks
Species Reactivity Zebrafish
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity chromatography
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt A0A2R8QPC9
Localization Cytoplasm (Mitochondria)
Applications Immunohistochemistry (FFPE) : 2-5 ug/ml
Limitations This Zebrafish Idh3b antibody is available for research use only.
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Description

Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial is an enzyme that in humans is encoded by the IDH3B gene. Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the beta subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Application Notes

Optimal dilution of the Zebrafish Idh3b antibody should be determined by the researcher.

Immunogen

An E.coli-derived zebrafish Idh3b recombinant protein (amino acids T33-D363) was used as the immunogen for the Zebrafish Idh3b antibody.

Storage

After reconstitution, the Zebrafish Idh3b antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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