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Home >> Antibodies >> Zebrafish Hsc70 Antibody / Hspa8

Zebrafish Hsc70 Antibody / Hspa8 (RZ1113)

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  Catalog No Formulation Size Price (USD)  
Image RZ1113 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 539
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IHC staining of FFPE zebrafish brain tissue with Zebrafish Hsc70 antibody, HRP secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE zebrafish brain tissue with Zebrafish Hsc70 antibody, HRP secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot analysis of Hsc70/HSPA8 protein using Hsc70/HSPA8 antibody and 1) zebrafish head, 2) female zebrafish viscera, 3) male zebrafish viscera, 4) whole female zebrafish, 5) whole male zebrafish and 6) zebrafish embryo tissue lysate. Predicted molecular weight ~71 kDa.
IHC staining of FFPE zebrafish retina tissue with Zebrafish Hsc70 antibody, HRP secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Availability 2-3 weeks
Species Reactivity Zebrafish
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity chromatography
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q90473
Localization Cytoplasm, cell membrane, nucleolus
Applications Western Blot : 0.5-1 ug/ml
Immunohistochemistry (FFPE) : 2-5 ug/ml
Limitations This Zebrafish Hsc70 antibody is available for research use only.
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Description

HSPA8 (heat shock 70kDa protein 8) also known as HSC70, HSC71, HSP73, HSPA10, FORMERLY, LAP1 or LPS-ASSOCIATED PROTEIN 1, is a heat shock protein that in humans is encoded by the HSPA8 gene. The HSPA8 gene contains 9 exons and spans 5 kb. The deduced HSPA8 protein has 646 amino acids and a predicted molecular mass of 70,899 Da. And the HSPA8 gene is mapped on 11q24.1. HSPA8 plays an important role in cells by transiently associating with nascent polypeptides to facilitate correct folding. HSP73 also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Rapid decay involves AU-rich binding protein AUF1, which complexes with heat-shock proteins HSC70 and HSP70, translation initiation factor EIF4G, and poly (A)-binding protein. In the absence of Il3, Hsc70 formed a complex with Hsp40 and Hip, and this complex, in association with Eif4g and Pabp, formed a high-stability complex with Bim mRNA that protected it from ribonucleases.

Application Notes

Optimal dilution of the Zebrafish Hsc70 antibody should be determined by the researcher.

Immunogen

An E.coli-derived zebrafish Hsc70/HSPA8 recombinant protein (amino acids K539-K601) was used as the immunogen for the Zebrafish Hsc70 antibody.

Storage

After reconstitution, the Zebrafish Hsc70 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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