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Home >> Antibodies >> Zebrafish Hnrnpd Antibody

Zebrafish Hnrnpd Antibody (RZ1021)

  Catalog No Formulation Size Price (USD)  
Image RZ1021 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 539
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Western blot testing of zebrafish embryo lysate with Zebrafish Hnrnpd antibody. Predicted molecular weight ~35 kDa.
Immunofluorescent analysis of Hnrnpd protein using Zebrafish Hnrnpd antibody (green) and zebrafish embryo tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE zebrafish brain tissue with Zebrafish Hnrnpd antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE zebrafish cerebellum tissue with Zebrafish Hnrnpd antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE zebrafish heart tissue with Zebrafish Hnrnpd antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE zebrafish colon tissue with Zebrafish Hnrnpd antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE zebrafish muscle tissue with Zebrafish Hnrnpd antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Availability 2-3 weeks
Species Reactivity Zebrafish
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity chromatography
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt A0A8M9QC46
Localization Cytoplasm, Nucleus
Applications Western Blot : 0.5-1 ug/ml
Immunohistochemistry (FFPE) : 2-5 ug/ml
Immunofluorescence : 5 ug/ml
Limitations This Zebrafish Hnrnpd antibody is available for research use only.
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Description

Heterogeneous nuclear ribonucleoprotein D0 (HNRNPD) also known as AU-rich element RNA-binding protein 1 (AUF1) is a protein that in humans is encoded by the HNRNPD gene. It is mapped to 4q21.22. This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. Alternative splicing of this gene results in four transcript variants.

Application Notes

Optimal dilution of the Zebrafish Hnrnpd antibody should be determined by the researcher.

Immunogen

An E.coli-derived zebrafish Hnrnpd recombinant protein (amino acids K43-N203) was used as the immunogen for the Zebrafish Hnrnpd antibody.

Storage

After reconstitution, the Zebrafish Hnrnpd antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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