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Home >> Antibodies >> Zebrafish Hmgb1 Antibody / Hmgb1a / Hmgb1b

Zebrafish Hmgb1 Antibody / Hmgb1a / Hmgb1b (RZ1154)

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  Catalog No Formulation Size Price (USD)  
Image RZ1154 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 539
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Immunohistochemical analysis of Hmgb1a/b protein using Zebrafish Hmgb1 antibody, HRP secondary and DAB substrate with paraffin-embedded zebrafish brain tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Immunohistochemical analysis of Hmgb1a/b protein using Zebrafish Hmgb1 antibody, HRP secondary and DAB substrate with paraffin-embedded zebrafish brain tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunohistochemical analysis of Hmgb1a/b protein using Zebrafish Hmgb1 antibody, HRP secondary and DAB substrate with paraffin-embedded zebrafish brain tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunohistochemical analysis of Hmgb1a/b protein using Zebrafish Hmgb1 antibody, HRP secondary and DAB substrate with paraffin-embedded zebrafish brain tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunohistochemical analysis of Hmgb1a/b protein using Zebrafish Hmgb1 antibody, HRP secondary and DAB substrate with paraffin-embedded zebrafish eye tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunohistochemical analysis of Hmgb1a/b protein using Zebrafish Hmgb1 antibody, HRP secondary and DAB substrate with paraffin-embedded zebrafish colon tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot analysis of Hmgb1a/b protein using Zebrafish Hmgb1 antibody and 1) zebrafish head, 2) whole female zebrafish, 3) whole male zebrafish and 4) zebrafish embryo tissue lysate. Predicted molecular weight ~23 kDa.
Availability 2-3 weeks
Species Reactivity Zebrafish
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity chromatography
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q6NX86 , X1WFI9
Localization Nuclear, cytoplasmic, cell membrane, extracellular
Applications Immunohistochemistry (FFPE) : 2-5 ug/ml
Western Blot : 0.5-1ug/ml
Limitations This Zebrafish Hmgb1 antibody is available for research use only.
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Description

Zebrafish Hmgb1 (High mobility group box 1) is a non-histone chromatin-binding protein that regulates DNA architecture and gene transcription. It functions in nucleosome stability, DNA repair, and transcriptional regulation by bending DNA and facilitating interactions with other proteins. Zebrafish have two isoforms, Hmgb1a and Hmgb1b, which share structural similarity but may have distinct expression patterns during development.

Beyond its nuclear role, Hmgb1 can act as an extracellular signaling molecule involved in inflammation, immune responses, and tissue repair. The human ortholog of zebrafish Hmgb1 is HMGB1, which is highly conserved and serves similar functions in chromatin dynamics and extracellular signaling. This evolutionary conservation highlights its importance in both normal physiology and disease research.

Using a high-quality Zebrafish Hmgb1 antibody enables accurate detection in applications such as western blot, immunohistochemistry, and immunofluorescence. A Zebrafish Hmgb1 antibody from NSJ Bioreagents ensures sensitivity and reproducibility for studies of chromatin remodeling, immune signaling, and developmental biology. Selecting the right Zebrafish Hmgb1 antibody is essential for generating reliable and consistent results.

Application Notes

Optimal dilution of the Zebrafish Hmgb1 antibody should be determined by the researcher.

Immunogen

An E.coli-derived zebrafish Hmgb1a/b recombinant protein (amino acids D123-K153) was used as the immunogen for the Zebrafish Hmgb1 antibody. This antibody will detect the a and b isoforms.

Storage

After reconstitution, the Zebrafish Hmgb1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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