Zebrafish Her4 Antibody / Her4.1 / Her4.2 / Her4.3 / Her4.5 (RZ1188)

Description

Her4 (Hairy-related 4, Transcription factor HES-5) is a basic helix-loop-helix (bHLH) transcriptional repressor belonging to the hairy/enhancer-of-split-related (Her/Hes) family of genes. In zebrafish (Danio rerio), Her4 is a downstream effector of the Notch signaling pathway and plays a central role in the regulation of neurogenesis and cell fate determination during embryonic development.

Her4 is prominently expressed in the developing central nervous system, where it maintains neural progenitor populations by inhibiting premature neuronal differentiation. By repressing proneural gene expression, Her4 ensures the proper timing and patterning of neuron formation. It also contributes to lateral inhibition, a process critical for the spatial organization of neural tissues.

Because of its well-characterized role in Notch-mediated signaling and neurodevelopment, zebrafish Her4 is frequently used as a molecular marker for neural progenitor cells and as a model to study stem cell maintenance, differentiation, and the impact of disrupted Notch signaling in developmental disorders and disease.

Application Notes

Optimal dilution of the Zebrafish Her4 antibody should be determined by the researcher.

Immunogen

E. coli-derived zebrafish Her4 recombinant protein (amino acids M1-W152) was used as the immunogen for the Zebrafish Her4 antibody. This antibody will detect isoforms 1 & 2 & 3 & 5.

Storage

After reconstitution, the Zebrafish Her4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.