• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Order
Home >> Antibodies >> Zebrafish H2ax Antibody / Histone H2A.X

Zebrafish H2ax Antibody / Histone H2A.X (RZ1125)

NSJ bio Image Pdf Image
  Catalog No Formulation Size Price (USD)  
Image RZ1125 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 539
Bulk quote request
IHC staining of FFPE zebrafish brain tissue with Zebrafish H2ax antibody, HRP secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
›
IHC staining of FFPE zebrafish brain tissue with Zebrafish H2ax antibody, HRP secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot analysis of Histone H2A.X/H2AFX protein using Zebrafish H2ax antibody and 1) zebrafish head, 2) whole female zebrafish, 3) whole male zebrafish and 4) zebrafish embryo tissue lysate. Predicted molecular weight ~15 kDa.
IHC staining of FFPE zebrafish muscle tissue with Zebrafish H2ax antibody, HRP secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE zebrafish liver tissue with Zebrafish H2ax antibody, HRP secondary and DAB substrate. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Availability 2-3 weeks
Species Reactivity Zebrafish
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity chromatography
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q7ZUY3
Localization Nuclear
Applications Western Blot : 0.5-1 ug/ml
Immunohistochemistry (FFPE) : 2-5 ug/ml
Limitations This Zebrafish H2ax antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Related Products

Description

H2A histone family member X (usually abbreviated as H2AX) is a type of histone protein from the H2A family encoded by the H2AFX gene. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.

Application Notes

Optimal dilution of the Zebrafish H2ax antibody should be determined by the researcher.

Immunogen

An E.coli-derived zebrafish Histone H2A.X/H2AFX recombinant protein (amino acids R4-T121) was used as the immunogen for the Zebrafish H2ax antibody.

Storage

After reconstitution, the Zebrafish H2ax antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Related Products

   1.   View all Histone H2A.X antibodies

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.