Zebrafish Flt1 Antibody / Vegfr1 / Vegf receptor 1 (RZ1183)

Description

Fms-related tyrosine kinase 1 (Flt1), also known as vascular endothelial growth factor receptor 1 (VEGFR-1), is a high-affinity receptor for members of the vascular endothelial growth factor (VEGF) family. In zebrafish (Danio rerio), the flt1 gene encodes a transmembrane receptor tyrosine kinase that plays a critical role in vascular development and angiogenesis.

Zebrafish Flt1 is expressed in endothelial cells and is involved in the regulation of blood vessel formation by modulating VEGF signaling. Unlike its counterpart Kdrl (zebrafish VEGFR-2), which promotes angiogenic signaling, Flt1 primarily functions as a decoy receptor that binds VEGF-A and VEGF-B with high affinity, thereby fine-tuning angiogenic responses during embryogenesis. Zebrafish also express a soluble form of Flt1 (sFlt1), which lacks the intracellular kinase domain and acts as a natural inhibitor of VEGF signaling.

Expression of flt1 in zebrafish is detected early during embryonic development, particularly in developing vasculature such as the dorsal aorta, cardinal vein, and intersegmental vessels. Disruption of flt1 leads to aberrant vessel patterning and excessive endothelial sprouting, highlighting its essential regulatory function in vascular morphogenesis.

Due to the optical transparency of zebrafish embryos and the availability of vascular reporter lines, zebrafish Flt1 serves as a powerful model for studying angiogenesis, vascular remodeling, and the mechanisms of VEGF signaling in vivo. It is also a valuable tool for investigating vascular defects, cancer biology, and responses to anti-angiogenic therapies.

Application Notes

Optimal dilution of the Zebrafish Flt1 antibody should be determined by the researcher.

Immunogen

E. coli-derived zebrafish Flt1 recombinant protein (amino acids L32-K1229) was used as the immunogen for the Zebrafish Flt1 antibody.

Storage

After reconstitution, the Zebrafish Flt1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.