Zebrafish Dnm1 Antibody / Dnm1a / Dnm1b / Dynamin (RZ1219)

Description

Dnm1, or dynamin one, is a neuron-specific member of the dynamin family of large GTPase enzymes that are involved in membrane remodeling processes such as endocytosis and vesicle trafficking. In zebrafish, Dnm1 plays a critical role in the nervous system by facilitating the recycling of synaptic vesicles and maintaining efficient neurotransmission.

Zebrafish express multiple dnm1 transcript variants that give rise to isoform a and isoform b, which may differ in their tissue distribution, regulatory elements, or functional properties. Both isoforms contribute to the dynamic regulation of synaptic membranes but may be expressed at different developmental stages or in distinct neuronal populations.

Dnm1 isoform a is typically expressed during early neural development and is enriched in regions of the brain with high synaptic activity. It supports the rapid and repeated cycles of vesicle fission that are essential for sustained neurotransmitter release.

Dnm1 isoform b may have overlapping or complementary roles to isoform a, potentially contributing to synaptic maintenance or plasticity in mature neurons. It may also be involved in neural differentiation and axonal growth during zebrafish development.

Because of its essential function in synaptic physiology, zebrafish Dnm1 is a widely used model for studying neural development, synaptic transmission, and neurological disorders associated with defects in vesicle recycling. It is particularly valuable in research on seizure susceptibility and developmental brain disorders, where Dnm1 mutations are known to have pathogenic effects in other vertebrates.

Application Notes

Optimal dilution of the Zebrafish Dnm1 antibody should be determined by the researcher.

Immunogen

E. coli-derived zebrafish Dnm1a/b recombinant protein (amino acids G614-D666) was used as the immunogen for the Zebrafish Dnm1 antibody. This antibody will detect the a & b isoforms.

Storage

After reconstitution, the Zebrafish Dnm1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.