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Home >> Antibodies >> Zebrafish Dnaja2 Antibody / Dnaja2a / Dnaja2b

Zebrafish Dnaja2 Antibody / Dnaja2a / Dnaja2b (RZ1146)

  Catalog No Formulation Size Price (USD)  
Image RZ1146 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 539
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Immunohistochemical analysis of DNAJA2a/b protein using DNAJA2 antibody and paraffin-embedded zebrafish pancreas tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot analysis of DNAJA2a/b protein using Zebrafish Dnaja2 antibody and zebrafish embryo tissue lysate. The predicted molecular weight of DNAJA2a/b is 46 kDa.
Immunohistochemical analysis of DNAJA2a/b protein using Zebrafish Dnaja2 antibody and paraffin-embedded zebrafish kidney tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunohistochemical analysis of DNAJA2a/b protein using Zebrafish Dnaja2 antibody and paraffin-embedded zebrafish brain tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunohistochemical analysis of DNAJA2a/b protein using Zebrafish Dnaja2 antibody and paraffin-embedded zebrafish colon tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Availability 2-3 weeks
Species Reactivity Zebrafish
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity chromatography
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q7ZUP5
Applications Western Blot : 0.5-1 ug/ml
Immunohistochemistry (FFPE) : 2-5 ug/ml
Limitations This Zebrafish Dnaja2 antibody is available for research use only.
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  • Applications : WB, IHC-P, FACS, Direct ELISA
    Reactivity : Human, Mouse, Rat

Description

DnaJ homolog subfamily A member 2 is a protein that in humans is encoded by the DNAJA2 gene. It is mapped to 16q11.2. The protein encoded by this gene belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain. The product of this gene works as a cochaperone of Hsp70s in protein folding and mitochondrial protein import in vitro.

Application Notes

Optimal dilution of the Zebrafish DNAJA2 antibody should be determined by the researcher.

Immunogen

An E.coli-derived zebrafish DNAJA2a/b recombinant protein (amino acids M1-Q230) was used as the immunogen for the Zebrafish Dnaja2 antibody. This antibody will detect the a and b isoforms.

Storage

After reconstitution, the Zebrafish Dnaja2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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