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Home >> Antibodies >> Toll-interacting protein Antibody / TOLLIP

Toll-interacting protein Antibody / TOLLIP (RQ7574)

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  Catalog No Formulation Size Price (USD)  
Image RQ7574 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 439
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Immunofluorescent staining of FFPE human A549 cells with Toll-interacting protein antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human A549 cells with Toll-interacting protein antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human RT4, 2) human U-2 OS, 3) human HeLa, 4) human HepG2, 5) rat brain, 6) rat liver, 7) mouse brain and 8) mouse liver tissue lysate with Toll-interacting protein antibody. Expected molecular weight ~30 kDa.
IHC staining of FFPE human prostate cancer tissue with Toll-interacting protein antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human cervical cancer tissue with Toll-interacting protein antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with Toll-interacting protein antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Flow cytometry testing of human HeLa cells with Toll-interacting protein antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Toll-interacting protein antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q9H0E2
Localization Cytoplasmic
Applications Western Blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence : 5ug/ml
Flow Cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This Toll-interacting protein antibody is available for research use only.
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Description

TOLLIP (TOLL-Interacting Protein) is an inhibitory adaptor protein that in humans is encoded by the TOLLIP gene. Lo et al.(2009) stated that the TOLLIP gene maps to chromosome 11. The mouse gene maps to chromosome 7. By western blot analysis of embryonic kidney cells, Burns et al.(2000) confirmed the binding of TOLLIP to IL1RAP, to a complex of IL1RAP-IL1R1, and to IL18R. Burns et al.(2000) proposed that IL1B stimulation induces aggregation of IL1Rs, recruitment of MYD88 followed by TOLLIP-IRAK complexes, and the phosphorylation of IRAK by MYD88. This leads to the dissociation of TOLLIP from IRAK, which can then transmit the IL1-induced signals.

Application Notes

Optimal dilution of the Toll-interacting protein antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids M1-P274) was used as the immunogen for the Toll-interacting protein antibody.

Storage

After reconstitution, the Toll-interacting protein antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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