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Home >> Antibodies >> Tissue Factor Antibody / CD142 / F3

Tissue Factor Antibody / CD142 / F3 [clone CD142/9195] (V5690)

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  Catalog No Formulation Size Price (USD)  
Image V5690-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 539
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V5690-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 249
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V5690SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
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IHC staining of FFPE human tonsil tissue with Tissue Factor antibody (clone CD142/9195). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human tonsil tissue with Tissue Factor antibody (clone CD142/9195). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung carcinoma tissue with Tissue Factor antibody (clone CD142/9195). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human A431 cell lysate with Tissue Factor antibody (clone CD142/9195). Expected molecular weight: 33-50 kDa depending on glycosylation level.
SDS-PAGE analysis of purified, BSA-free Tissue Factor antibody (clone CD142/9195) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name CD142/9195
Purity Protein G affinity
UniProt P13726
Localization Secreted, membrane
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Western Blot : 2-4ug/ml
Limitations This Tissue Factor antibody is available for research use only.
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Description

Hemostasis following tissue injury involves the deployment of essential plasma procoagulants (Prothrombin and Factors X, IX, V and VIII), which are involved in a blood coagulation cascade leading to the formation of insoluble fibrin clots and the promotion of platelet aggregation. Coagulation Factor V (Factor V, FV, proaccelerin, labile factor) is a 2,196 amino acid, single chain glycoprotein that is cleaved by Thrombin to yield an active, Ca2+-dependent dimer that is essential to the blood coagulation cascade. Together with catalytic Factor Xa and Ca2+ on the surface of platelets or endothelial cells, Factor Va coordinates into a prothrombinase complex, which mediates proteolysis of Prothrombin into active Thrombin. Tissue factor (TF), also designated coagulation Factor III) is a cell surface glycoprotein that enables cells to initiate blood coagulation cascades. It functions as a high-affinity receptor for coagulation Factor VII.

Application Notes

Optimal dilution of the Tissue Factor antibody should be determined by the researcher.

Immunogen

A portion of amino acids 50-250 from human F3 protein was used as the immunogen for the Tissue Factor antibody.

Storage

Aliquot the Tissue Factor antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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