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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
SPRY3 Antibody / Sprouty RTK signaling antagonist 3 (FY13384)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
FY13384 | Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml | 100 ug | 439 |
SPRY3 antibody detects Sprouty RTK signaling antagonist 3, a negative regulator of receptor tyrosine kinase (RTK) signaling encoded by the SPRY3 gene located on the X and Y chromosomes (Xp22.33/Yp11.3). SPRY3 belongs to the sprouty family of cytoplasmic signaling modulators that fine-tune growth factor receptor signaling, particularly pathways initiated by fibroblast growth factors (FGFs), epidermal growth factor (EGF), and vascular endothelial growth factor (VEGF). It is expressed in brain, lung, placenta, and testes, where it regulates cell growth, differentiation, and angiogenesis.
Structurally, SPRY3 contains a conserved cysteine-rich C-terminal domain responsible for membrane association and a variable N-terminal region that mediates interaction with adapter proteins such as GRB2 and c-CBL. It belongs to the sprouty protein family (SPRY1-4), known for modulating Ras/MAPK signaling by interfering with RAF activation. Co-localization studies show SPRY3 associated with endosomal membranes and perinuclear regions, reflecting its regulatory role at signal transduction interfaces.
Functionally, SPRY3 acts as an inhibitor of the Ras/MAPK pathway, suppressing excessive signaling following receptor activation. It prevents cell overproliferation and supports proper developmental patterning. In neurons, SPRY3 regulates axonal branching and synaptic plasticity, while in endothelial cells it modulates angiogenic responses to VEGF. SPRY3 also interacts with E3 ubiquitin ligases such as c-CBL, influencing receptor internalization and degradation. Known downstream pathways include ERK1/2, PI3K-AKT, and Rho family GTPase signaling.
Expression of SPRY3 is developmentally regulated, with high levels observed in fetal brain and during vascular remodeling. Dysregulation or loss of SPRY3 has been linked to tumorigenesis, impaired angiogenesis, and developmental disorders. Deletion of SPRY3 in the pseudoautosomal region may contribute to neurodevelopmental phenotypes in certain chromosomal abnormalities. Pathway associations include growth factor receptor signaling, neuronal differentiation, and vascular development. In disease models, SPRY3 acts as a tumor suppressor through attenuation of mitogenic signaling.
Immunohistochemical staining using SPRY3 antibody reveals cytoplasmic localization in neurons, endothelial cells, and epithelial tissues. The SPRY3 antibody from NSJ Bioreagents is a valuable tool for studying growth factor signaling regulation, angiogenesis, and neurodevelopmental pathways.
Optimal dilution of the SPRY3 antibody should be determined by the researcher.
E.coli-derived human SPRY3 recombinant protein (Position: M1-R162) was used as the immunogen for the SPRY3 antibody.
After reconstitution, the SPRY3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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