SLC17A6 Antibody / VGLUT2 / Vesicular glutamate transporter 2 (FY12581)

Western blot analysis of VGLUT2/SLC17A6 using anti-SLC17A6 antibody. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC17A6 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. Western blot analysis of cell lysates probed with anti-SLC17A6 shows a major band at the expected ~64 kDa and minor lower bands corresponding to partially deglycosylated or processed forms of the N-glycosylated SLC17A6/VGLUT2 protein