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Home >> Antibodies >> Recombinant Tripartite motif-containing protein 29 Antibody / TRIM29

Recombinant Tripartite motif-containing protein 29 Antibody / TRIM29 [clone TRIM29/9257R] (V5598)

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  Catalog No Formulation Size Price (USD)  
Image V5598-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 539
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V5598-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 249
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V5598SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
Microvalidated Recrabbitmono
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IHC staining of FFPE human esophagus tissue with recombinant Tripartite motif-containing protein 29 antibody (clone TRIM29/9257R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human esophagus tissue with recombinant Tripartite motif-containing protein 29 antibody (clone TRIM29/9257R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant Tripartite motif-containing protein 29 antibody (clone TRIM29/9257R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name TRIM29/9257R
Purity Protein A/G affinity
UniProt Q14134
Localization Cytoplasm, Cell membrane
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant Tripartite motif-containing protein 29 antibody is available for research use only.
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Description

Tripartite motif-containing protein 29 (TRIM29) interacts with the intermediate filament protein vimentin, a substrate for the PKC family of protein kinases, and with hPKCI-1, an inhibitor of the PKCs. TRIM29 protein contains both zinc finger and leucine zipper motifs, suggesting that the it may form homodimers and possibly associate with DNA. High expression of TRIM29 has been reported in gastric cancer and pancreatic cancer, and correlates with enhanced tumor growth and lymph node metastasis. TRIM29 is also able to distinguish lung squamous cell carcinoma from lung adenocarcinoma with ~90% positive accuracy, when used in a panel with TTF-1, p63, CK5/6, and Napsin-A antibodies.

Application Notes

Optimal dilution of the recombinant Tripartite motif-containing protein 29 antibody should be determined by the researcher.

Immunogen

A recombinant fragment (within amino acids 1-200) of human recombinant Tripartite motif-containing protein 29 protein was used as the immunogen for the TRIM29 antibody.

Storage

Aliquot the recombinant Tripartite motif-containing protein 29 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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