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Home >> Antibodies >> Recombinant TAG-72 Antibody

Recombinant TAG-72 Antibody [clone rTAG72/9132] (V5379)

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  Catalog No Formulation Size Price (USD)  
Image V5379-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 539
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V5379-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 249
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V5379SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
Microvalidated Recmousemono
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IHC staining of FFPE human colon tissue with recombinant TAG-72 antibody (clone rTAG72/9132). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human colon tissue with recombinant TAG-72 antibody (clone rTAG72/9132). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant TAG-72 antibody (clone rTAG72/9132) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG1, kappa
Clone Name rTAG72/9132
Purity Protein A/G affinity
Localization Cytoplasm, Cell Surface
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant TAG-72 antibody is available for research use only.
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Description

Recognizes an oncofetal antigen of 220kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This MAb defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80% sensitivity and 93% specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma.

Application Notes

Optimal dilution of the recombinant TAG-72 antibody should be determined by the researcher.

Immunogen

A membrane-enriched fraction of a human breast carcinoma liver metastasis was used as the immunogen for the recombinant TAG-72 antibody.

Storage

Aliquot the recombinant TAG-72 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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