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Home >> Antibodies >> Recombinant PLAP Antibody / Placental Alkaline Phosphatase

Recombinant PLAP Antibody / Placental Alkaline Phosphatase [clone ALPP/8112R] (V5429)

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  Catalog No Formulation Size Price (USD)  
Image V5429-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 539
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V5429-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 249
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V5429SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
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IHC staining of FFPE human placental tissue with recombinant PLAP antibody (clone ALPP/8112R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human placental tissue with recombinant PLAP antibody (clone ALPP/8112R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placental tissue with recombinant PLAP antibody (clone ALPP/8112R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant PLAP antibody (clone ALPP/8112R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name ALPP/8112R
Purity Protein A/G affinity
UniProt P05187
Localization Cytoplasm, Cell membrane
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant PLAP antibody is available for research use only.
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Description

Reacts with a 70kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease.

Application Notes

Optimal dilution of the recombinant PLAP antibody should be determined by the researcher.

Immunogen

Recombinant full-length human Placental Alkaline Phosphatase protein was used as the immunogen for the recombinant PLAP antibody.

Storage

Aliquot the recombinant PLAP antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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