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Home >> Antibodies >> Recombinant H3K9ac Antibody

Recombinant H3K9ac Antibody [clone RM161] (R20208)

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  Catalog No Formulation Size Price (USD)  
Image R20208-100UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ug 485
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R20208-25UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 25 ug 249
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Western blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate using recombinant H3K9ac antibody at 0.25 ug/ml showed a band of Histone H3 acetylated at Lysine 9 in treated HeLa cells.
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Western blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate using recombinant H3K9ac antibody at 0.25 ug/ml showed a band of Histone H3 acetylated at Lysine 9 in treated HeLa cells.
Sandwich ELISA with acetylated Histone H3 at Lys 9 using HeLa whole cell lysate, treated or untreated with sodium butyrate, anti-H3CT (RM188, 1 ug/ml) as the capture and biotinylated recombinant H3K9ac antibody (RM161, 1 ug/ml) as the detect.
ChIP performed on HeLa cells using recombinant H3K9ac antibody (5ug). Real-time PCR was performed using primers specific to the gene indicated.
ICC/IF testing of HeLa cells treated with sodium butyrate using recombinant H3K9ac antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
ICC testing of human HepG2 cells with recombinant H3K9ac antibody.
The recombinant H3K9ac antibody specifically reacts to Histone H3 acetylated at Lysine 9 (K9ac). No cross reactivity with acetylated Lysine 4/14/18/23/27/36/56/79/122 in Histone H3.
Availability 1-3 business days
Species Reactivity All Species
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM161
Purity Protein A purified from animal origin-free supernatant
UniProt P84243
Gene ID 8350
Applications Western Blot : 0.25-1ug/ml
Immunocytochemistry : 0.5-2ug/ml
ChIP : 2ug/ml-10ug/mg of lysate
Immunohistochemistry : 0.1-1ug/ml (1)
ELISA : 0.5-1ug/ml
Limitations This recombinant H3K9ac antibody is available for research use only.
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Description

This recombinant H3K9ac antibody reacts to Histone H3 acetylated at Lysine 9 (K9ac). No cross reactivity with other acetylated Lysines in histone H3.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant H3K9ac antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Immunogen

An acetyl-peptide corresponding to the Acetyl-Histone H3 (Lys9) was used as the immunogen for this recombinant H3K9ac antibody.

Storage

Store the recombinant H3K9ac antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Related Products

   1.   View all Recombinant antibodies

   2.   View all H3K9ac antibodies

   3.   View all Histone H3 antibodies

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