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Home >> Antibodies >> Recombinant H3K23ac Antibody

Recombinant H3K23ac Antibody [clone RM169] (R20229)

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  Catalog No Formulation Size Price (USD)  
Image R20229-100UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 100 ug 485
Image
R20229-25UG 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide 25 ug 249
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Western blot test of acid extracts of HeLa cells non-treated (1) or treated with sodium butyrate (2) and recombinant Histone H3.3 (3), using recombinant H3K23ac antibody at 1 ug/ml, showed a band of Histone H3 acetylated at Lysine 23 in treated HeLa cells.
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Western blot test of acid extracts of HeLa cells non-treated (1) or treated with sodium butyrate (2) and recombinant Histone H3.3 (3), using recombinant H3K23ac antibody at 1 ug/ml, showed a band of Histone H3 acetylated at Lysine 23 in treated HeLa cells.
This recombinant H3K23ac antibody specifically reacts to Histone H3 acetylated at Lysine 23 (K23ac). No cross reactivity with unmodified Lysine 23 (K23 ctrl) or other acetylated Lysines.
ICC/IF staining of HeLa cells treated with sodium butyrate using recombinant H3K23ac antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Availability 1-3 business days
Species Reactivity All Species
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name RM169
Purity Protein A purified from animal origin-free supernatant
UniProt P84243
Gene ID 8350
Applications Western Blot : 0.5-2ug/ml
ELISA : 0.5-1ug/ml
Immunocytochemistry : 1-2ug/ml
Limitations This recombinant H3K23ac antibody is available for research use only.
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Description

This recombinant H3K23ac antibody reacts to Histone H3 acetylated at Lysine 23 (K23ac). No cross reactivity with other acetylated Lysines in Histone H3.

Application Notes

The stated application concentrations are suggested starting points. Titration of the recombinant H3K23ac antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys23) was used as the immunogen for this recombinant H3K23ac antibody.

Storage

Store the recombinant H3K23ac antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Related Products

   1.   View all Recombinant antibodies

   2.   View all H3K23ac antibodies

   3.   View all Histone H3 antibodies

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