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Home >> Antibodies >> Recombinant FLI1 Antibody

Recombinant FLI1 Antibody [clone rFLI1/9614] (V5577)

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  Catalog No Formulation Size Price (USD)  
Image V5577-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 539
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V5577-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 249
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V5577SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
Microvalidated Recmousemono
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IHC staining of FFPE human Ewing Sarcoma tissue with recombinant Fli1 antibody (clone rFLI1/9614). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human Ewing Sarcoma tissue with recombinant Fli1 antibody (clone rFLI1/9614). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant FLI1 antibody (clone rFLI1/9614) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Mouse Monoclonal
Isotype Mouse IgG2b, kappa
Clone Name rFLI1/9614
Purity Protein A/G affinity
UniProt Q01543
Localization Nucleus
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This recombinant FLI1 antibody is available for research use only.
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Description

Recognizes a protein of 51kDa, which is identified as FLI1. This protein, a member of the ETS family of DNA binding transcription factors, is involved in cellular proliferation and tumorigenesis. Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. Members of the Ets gene family share a highly conserved carboxy-terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. Approximately 90% of Ewing s Sarcoma (EWS) / Primitive Neuroectodermal Tumors (PNET) have a specific translocation, t(11;22)(q24;q12), which results in fusion of EWS to Fli-1, and production of an EWS-Fli-1 fusion protein. Among normal tissues only endothelial cells and small lymphocytes express Fli-1. This protein is expressed in majority of vascular tumors including angiosarcomas, hemangioendotheliomas, hemangiomas, and Kaposi s Sarcomas. High sensitivity and specificity of Fli-1 equals to or exceeds that of the established vascular markers like CD31, CD34, and Factor VIII.

Application Notes

Optimal dilution of the recombinant FLI1 antibody should be determined by the researcher.

Immunogen

A recombinant fragment (within amino acids 200-400) of human FLI1 protein was used as the immunogen for the recombinant FLI1 antibody.

Storage

Aliquot the recombinant FLI1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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