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Home >> Antibodies >> Recombinant Epidermal growth factor receptor Antibody / EGFR

Recombinant Epidermal growth factor receptor Antibody / EGFR [clone EGFR/9166R] (V5391)

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  Catalog No Formulation Size Price (USD)  
Image V5391-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 539
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V5391-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 249
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V5391SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
Microvalidated Recrabbitmono
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Western blot testing of human A431 cell lysate with recombinant Epidermal growth factor receptor antibody (clone EGFR/9166R). Expected molecular weight: 134-180 kDa depending on glycosylation level.
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Western blot testing of human A431 cell lysate with recombinant Epidermal growth factor receptor antibody (clone EGFR/9166R). Expected molecular weight: 134-180 kDa depending on glycosylation level.
IHC staining of FFPE human placenta tissue with recombinant Epidermal growth factor receptor antibody (clone EGFR/9166R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant Epidermal growth factor receptor antibody (clone EGFR/9166R) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name EGFR/9166R
Purity Protein A/G affinity
UniProt P00533
Localization Cell Surface
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Western Blot : 2-4ug/ml
Limitations This recombinant Epidermal growth factor receptor antibody is available for research use only.
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Description

This MAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin.EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

Application Notes

Optimal dilution of the recombinant Epidermal growth factor receptor antibody should be determined by the researcher.

Immunogen

A recombinant fragment (within amino acids 300-500) of human EGFR protein was used as the immunogen for the recombinant Epidermal growth factor receptor antibody.

Storage

Aliquot the recombinant Epidermal growth factor receptor antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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