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Home >> Antibodies >> Recombinant CK8/18 Antibody / Cytokeratin 8/18

Recombinant CK8/18 Antibody / Cytokeratin 8/18 [clone KRT8.18/6579R] (V5434)

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  Catalog No Formulation Size Price (USD)  
Image V5434-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 539
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V5434-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 249
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V5434SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
Microvalidated Recrabbitmono
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IHC staining of FFPE human colon tissue with recombinant CK8/18 antibody (KRT8.18/6579R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human colon tissue with recombinant CK8/18 antibody (KRT8.18/6579R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colon tissue with recombinant CK8/18 antibody (KRT8.18/6579R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Recombinant Rabbit Monoclonal
Isotype Rabbit IgG, kappa
Clone Name KRT8.18/6579R
Purity Protein A/G affinity
UniProt P05787 , P05783
Localization Cytoplasm, Nucleus
Applications Flow Cytometry : 1-2ug/million cells
Immunofluorescence : 1-3ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Western Blot : 2-4ug/ml
Limitations This recombinant CK8/18 antibody is available for research use only.
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Description

Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.Immunohistochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3.

Application Notes

Optimal dilution of the recombinant CK8/18 antibody should be determined by the researcher.

Immunogen

Recombinant full-length human CK8 and CK18 proteins were used as the immunogen for the recombinant CK8/18 antibody.

Storage

Aliquot the recombinant CK8/18 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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