Recombinant CD19 Antibody [clone CD19/4260R] (V5821)

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using recombinant CD19 antibody (clone CD19/4260R). These results demonstrate the foremost specificity of the CD19/4260R mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

IHC staining of FFPE human tonsil tissue with recombinant CD19 antibody (clone CD19/4260R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human tonsil tissue lysate with recombinant CD19 antibody (clone CD19/4260R). Expected molecular weight: 60~100 kDa depending on glycosylation level.

Western blot testing of human Raji cell lysate with recombinant CD19 antibody (clone CD19/4260R). Expected molecular weight: 60~100 kDa depending on glycosylation level.

SDS-PAGE analysis of purified, BSA-free recombinant CD19 antibody (clone CD19/4260R) as confirmation of integrity and purity.