Recombinant ACTN2 Antibody / Alpha Actinin 2 / Sarcomeric Alpha Actinin [clone ACTN2/7039R] (V9421)

IHC staining of FFPE human skeletal muscle tissue with recombinant recombinant ACTN2 antibody (clone ACTN2/7039R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human heart tissue with recombinant recombinant ACTN2 antibody (clone ACTN2/7039R). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using recombinant ACTN2 antibody (clone ACTN2/7039R). These results demonstrate the foremost specificity of the ACTN2/7039R mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free recombinant ACTN2 antibody (clone ACTN2/7039R) as confirmation of integrity and purity.