• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Home >> Antibodies >> Protocadherin alpha 12 Antibody / PCDHA12

Protocadherin alpha 12 Antibody / PCDHA12 (RQ8711)

NSJ bio Image Pdf Image
  Catalog No Formulation Size Price (USD)  
Image RQ8711 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 439
Bulk quote request
Flow cytometry testing of fixed human U-2 OS cells with Protocadherin alpha 12 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Protocadherin alpha 12 antibody.
›
Flow cytometry testing of fixed human U-2 OS cells with Protocadherin alpha 12 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Protocadherin alpha 12 antibody.
Western blot testing of human 1) RT4 and 2) MCF7 cell lysate with Protocadherin alpha 12 antibody. Predicted molecular weight ~101 kDa.
Availability 1-3 days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity chromatography
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q9UN75
Applications Western Blot : 1-2ug/ml
Flow Cytometry : 1-3ug/million cells
ELISA : 0.1-0.5ug/ml
Limitations This Protocadherin alpha 12 antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Description

Protocadherin alpha-12 is a protein that in humans is encoded by the PCDHA12 gene. This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined.

Application Notes

Optimal dilution of the Protocadherin alpha 12 antibody should be determined by the researcher.

Immunogen

An E.coli-derived human recombinant protein (amino acids K219-V365) was used as the immunogen for the Protocadherin alpha 12 antibody.

Storage

After reconstitution, the Protocadherin alpha 12 Antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Related Products

   1.   View all PCDHA12 antibodies

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.