- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
POLR2A Antibody / RNA polymerase II subunit RPB1 [clone CTD 4H8] (V7452)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
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V7452-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 100 ug | 539 | |
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V7452-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide | 20 ug | 249 | |
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V7452SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 539 | |
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V7452IHC-7ML | Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* | 7 ml | 539 |
| Availability | 1-3 business days |
| Species Reactivity | Human, Mouse, Rat |
| Format | Purified |
| Clonality | Monoclonal (mouse origin) |
| Isotype | Mouse IgG1, kappa |
| Clone Name | CTD 4H8 |
| Purity | Protein G affinity chromatography |
| UniProt | P24928 |
| Localization | Nuclear |
| Applications | Flow Cytometry : 1-2ug/10^6 cells Immunofluorescence : 1-2ug/ml Western Blot : 1-2ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT |
| Limitations | This POLR2A antibody is available for research use only. |
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POLR2A antibody detects the largest subunit of RNA polymerase II, encoded by the POLR2A gene. RNA polymerase II is responsible for transcribing all protein-coding genes, as well as many non-coding RNAs. POLR2A contains a unique C-terminal domain (CTD) with heptad repeats that undergo dynamic phosphorylation, regulating transcription initiation, elongation, and termination. Because transcriptional regulation underlies nearly all aspects of cell biology, POLR2A antibody is indispensable in molecular biology, epigenetics, and cancer research.
The POLR2A subunit serves as a central platform for assembly of transcription factors, elongation factors, and RNA processing machinery. The phosphorylation state of the CTD determines the recruitment of capping enzymes, splicing factors, and polyadenylation complexes. This sequential modification of CTD repeats acts as a molecular code, coordinating transcription with co-transcriptional RNA processing. Dysregulation of POLR2A activity has profound consequences, contributing to developmental disorders and cancer.
The POLR2A antibody clone CTD 4H8 specifically recognizes epitopes within the C-terminal domain. Clone CTD 4H8 has been widely cited in peer-reviewed studies that investigate transcriptional dynamics, chromatin regulation, and polymerase pausing. Its consistent performance makes it suitable for Western blotting, chromatin immunoprecipitation, and immunofluorescence, where precise detection of RNA polymerase II activity is required.
Research using clone CTD 4H8 has demonstrated how POLR2A phosphorylation patterns reflect transcriptional states. Detection of hypophosphorylated versus hyperphosphorylated forms allows investigators to distinguish between initiation and elongation phases of transcription. This antibody has also supported studies exploring how transcription is altered in cancer, where mutations in regulatory factors disrupt polymerase function. By tracking POLR2A status, researchers gain insight into fundamental gene expression mechanisms and their disease-related alterations.
NSJ Bioreagents supplies this POLR2A antibody to support transcription research, epigenetics, and oncology. Alternate terms include RNA polymerase II subunit A antibody, RPB1 antibody, CTD repeat antibody, RNA polymerase II large subunit antibody, and transcriptional machinery antibody.
Optimal dilution of the recombinant POLR2A antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
The immunogen for this POLR2A antibody was 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-S5.
Store the POLR2A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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