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Home >> Antibodies >> Podoplanin Antibody

Podoplanin Antibody [clone D2-40] (V5650)

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  Catalog No Formulation Size Price (USD)  
Image V5650-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 539
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V5650-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 249
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V5650SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
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IHC staining of FFPE human tonsil tissue with Podoplanin antibody (clone D2-40). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with Podoplanin antibody (clone D2-40). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name D2-40
Purity Protein G affinity
UniProt Q86YL7
Localization Cytoplasm, Membrane
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Limitations This Podoplanin antibody is available for research use only.
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Description

Podoplanin is a transmembrane mucoprotein (38 kDa) recognized by the D2-40 monoclonal antibody. Podoplanin is specifically expressed in the endothelium of lymphatic capillaries but not in the blood vasculature. In normal skin and kidney, podoplanin is co-localized with VEGFR3/FLT4, another marker for lymphatic endothelial cells. Podoplanin is selectively expressed in lymphatic endothelium as well as Lymphangiomas, Kaposi’s Sarcomas and in subset Angiosarcomas with probable lymphatic differentiation. Podoplanin has also been shown to be expressed in Epithelioid Mesotheliomas, Hemangioblastomas and Seminomas.

Application Notes

Optimal dilution of the Podoplanin antibody should be determined by the researcher.

Immunogen

Resected tissue from dysgeminoma of the ovary was used as the immunogen for the Podoplanin antibody.

Storage

Aliquot the Podoplanin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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