PLB Antibody / Phospholamban / PLN (RQ6787)

Description

PLB (Phospholamban) is a small transmembrane protein located in the sarcoplasmic reticulum (SR) of cardiac and skeletal muscle cells. It plays a critical role in regulating calcium cycling by modulating the activity of the SR Ca²⁺-ATPase (SERCA). In its unphosphorylated state, PLB inhibits SERCA function, reducing calcium uptake into the SR, while phosphorylation of PLB relieves this inhibition, enhancing calcium reuptake and promoting efficient muscle relaxation.

Through its control of SERCA activity, PLB contributes to the fine-tuning of excitation–contraction coupling and influences both cardiac contractility and muscle performance. Its phosphorylation status and expression levels are often measured in studies of muscle physiology, calcium signaling, and cardiac function under varying experimental conditions.

The PLB antibody is a reliable tool for detecting endogenous phospholamban in applications such as western blot, immunohistochemistry, and immunofluorescence. Researchers use the PLB antibody from NSJ Bioreagents to quantify protein abundance, assess subcellular localization, and evaluate phosphorylation-related changes in calcium handling. With high specificity and consistent performance, the PLB antibody supports detailed investigations into muscle physiology, calcium regulation, and sarcoplasmic reticulum function.

Application Notes

Optimal dilution of the PLB antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids M1-C36) was used as the immunogen for the PLB antibody.

Storage

After reconstitution, the PLB antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.